Re: ROI post processing help: split in two equal surfaces

Posted by Nathaniel Ryckman on Mar 30, 2011; 6:24pm
URL: http://imagej.273.s1.nabble.com/ROI-post-processing-help-split-in-two-equal-surfaces-tp3685206p3685209.html

You could iteratively split the particle, measure the areas of the two new particles, and then save the results if the ratio between the two areas is ~1. You could do a binary search for the correct axis, using the left and right boundary of the original particle as limits.

A couple things to note:
1) I think you are only using vertical axis to split the particle? If you rotate the axis, you would have more opportunities to find a good axis, although, I wouldn't think that would be worth the trouble.
2) Why is dividing the particle so essential if you don't mind changing where the particle is split? Choosing an axis that would give the 2 new particles roughly equal area would essentially be changing their shapes, right?
3) I'm not really understanding the nature of the problem that well, but, if I understand correctly, I would just use Mean Gray Value instead of Integrated Density. I think that's maybe what you really are interested in.

Here is more information about what each of the measurement commands do:
http://rsb.info.nih.gov/ij/docs/menus/analyze.html