declump those buggers

Posted by Matthias Kirsch on Mar 21, 2011; 8:19pm
URL: http://imagej.273.s1.nabble.com/declump-those-buggers-tp3685220.html

dear all,

I am struggling with automatic image analysis. One of the first stumbling point seems to be segmenting DAPI-stained nuclei in fluorescence images. If this is not working reliably, the whole point of processing large numbers of images is obsolete. Attached are examples of such clumps (there are many more). If segmentation cannot separate them, it would be helpful to have at least some way of eliminating false results by just clicking on them, or even better, have all identified particles presented in a graph with freely chosen measurement as parameters as axes. Then one could click on the individual points in the graph and see the corresponding cell in the image and then decide whether to keep or discaed it. This should also work the other way round, i.e. click on a cell in the image and see the corresponding point in the graph. Similarily, it would be most helpful to be able do somethig like gating in FACS, i.e. set limits to certain parameters and see, which cells fulill them.

I know that this is asking a lot, but I think providing this kind of routines in an easy to use plugin would make ImageJ even more attractive and competitive than it already is. Of course this applies only for 'Non-Cracks'.

Any help would be welcome. Further questions alnosr guaranteed.

Cheers

P.S.: Can only insert jpeg images
Matthias