http://imagej.273.s1.nabble.com/declump-those-buggers-tp3685220p3685230.html
P.S. You might want to try out different "Analyze Particles" settings for the size and circularity options to the to find the results that best suit your needs:
run("Analyze Particles...", "size=200-500 circularity=0.70-1.00 show=Outlines display exclude clear add");
> dear all,
>
> I am struggling with automatic image analysis. One of the first stumbling
> point seems to be segmenting DAPI-stained nuclei in fluorescence images. If
> this is not working reliably, the whole point of processing large numbers of
> images is obsolete. Attached are examples of such clumps (there are many
> more). If segmentation cannot separate them, it would be helpful to have at
> least some way of eliminating false results by just clicking on them, or
> even better, have all identified particles presented in a graph with freely
> chosen measurement as parameters as axes. Then one could click on the
> individual points in the graph and see the corresponding cell in the image
> and then decide whether to keep or discaed it. This should also work the
> other way round, i.e. click on a cell in the image and see the corresponding
> point in the graph. Similarily, it would be most helpful to be able do
> somethig like gating in FACS, i.e. set limits to certain parameters and see,
> which cells fulill them.
>
> I know that this is asking a lot, but I think providing this kind of
> routines in an easy to use plugin would make ImageJ even more attractive and
> competitive than it already is. Of course this applies only for
> 'Non-Cracks'.
>
> Any help would be welcome. Further questions alnosr guaranteed.
>
> Cheers
>
> P.S.: Can only insert jpeg images
> Matthias
http://imagej.588099.n2.nabble.com/file/n6193826/RGB_%28blue%29.jpg
>
> --
> View this message in context:
http://imagej.588099.n2.nabble.com/declump-those-buggers-tp6193826p6193826.html> Sent from the ImageJ mailing list archive at Nabble.com.