Analyzing adjacent ROIs

Posted by Alexander C. Taylor on Mar 23, 2011; 10:33pm
URL: http://imagej.273.s1.nabble.com/Analyzing-adjacent-ROIs-tp3685285.html

Hey folks, I'm new here and I'd appreciate some help if anyone has a few minutes. I hope I'm posting this in the correct area.

I have some long bone sections with staining, and I'm trying to find out if there's a gradient, as in more staining towards the center compared with toward the periphery.

From what I can find, there seems to be no option to give ImageJ a rectangular ROI and ask it to compute staining gradients, so I've been trying to find a way to align multiple small rectangles: [][][][][][][][] from the outer edge of the bone in the image to the center of the bone. These rectangles would need to be uniform and perfectly aligned, and I'd analyze each for total staining to see if this increased in a given direction. These rectangles would be at an angle, since I have to go from a certain spot on the outer edge of the bone sections.

Is there any way to achieve this, or another way to determine something like this? I'm at a loss.

Thanks, folks. -Alex