Re: Analyzing adjacent ROIs

Posted by Michael Doube on Mar 25, 2011; 10:27am
URL: http://imagej.273.s1.nabble.com/Analyzing-adjacent-ROIs-tp3685285p3685287.html

> I'm just trying to get a not-terribly-sophisticated measurement
> of whether staining intensity has some sort of gradient from the
> middle of the marrow to the edges or vice versa,
A really simple way to do this is simply to draw a line across the
marrow, (double click line tool icon to change line width), then hit [k]
on your keyboard to get a profile plot of pixel value vs. distance along
line.  A gradient will show up as a slope in the line. This will of
course also include the trabeculae if you leave the images as they are;
you might have to do some segmentation before a profile plot gives you
results on marrow only.

Michael