Posted by
Michael Doube on
Mar 24, 2011; 3:23pm
URL: http://imagej.273.s1.nabble.com/Analyzing-adjacent-ROIs-tp3685285p3685289.html
Hi Alex
> Thanks for your reply, Michael! Yes, the sections are fairly annular-
> and yes, they're sections of long bone. Here's a link to an image
> similar to the ones I'm dealing with:
>
http://decembermouse.webuda.com/Sample/sample.jpgThanks for the sample image.
A more immediate issue appears to be how you plan to measure 'staining'
at all, even before attempting to show any spatial trend, and what that
measurement might seek to represent. There are quite a few problems
with measuring the strength or amount of histological staining, which
get discussed on this list from time to time.
The morphology of the section shows shrinkage and sectioning artefact,
uneven staining and tearing, so you'd have to work around that as well
(some dark stripes look like they are due to knife chatter for example).
It looks like the most prominent staining is the edge of the marrow
where it has come away from the periosteal surface.
So while it's possible to proceed with some kind of spatial analysis,
I'm not convinced that your image actually holds useful information of
the kind you are seeking.
Michael