3d Skeletonization/Analysis of Vasculature

Posted by John87h on Mar 24, 2011; 9:35pm
URL: http://imagej.273.s1.nabble.com/3d-Skeletonization-Analysis-of-Vasculature-tp3685299.html

I have 3d images of maturing zebrafish vasculature tagged with green fluorescent protein, taken with a confocal microscope.  I am running into problems with the analysis (measurement of length, area, and branching) of these images.  I have been trying the skeletonize and analyzeskeleton plugins on ImageJ, but I cannot generate good 3d skeletonizations.  I have been importing the image stacks as binary .tif image sequences, and I have tried setting different thresholds for the image to no avail.  I would appreciate any advice.

- John