Re: merging chanels?

Posted by Michael Schmid on Mar 20, 2011; 7:43pm
URL: http://imagej.273.s1.nabble.com/merging-chanels-tp3685340p3685341.html

Hi Michael,

maybe you have changed the Edit>Options>Conversions 'Scale when
converting' settings?

Michael
_______________________________________________________________________

On Sat, March 19, 2011 19:11, Cammer, Michael wrote:

> Has there been a change in merging channels?
>
> We used to adjust the contrast of 16 bit images to look the way we wanted
> them onthe screen and then merge them with Image > Color > Merge
> Channels... with composite off and Keep Source Images on.  If we split the
> resultant image later, we got 8 bit versions of the grayscale images we
> saw onthe monitor.
>
> Today, using 1.45 with 32 bit Vista, the merged images are being contrast
> adjusted to look brighter, including features saturated, and splitting the
> channels shows that this was applied to the grayscale during the merge
> process.
>
> For now we're converting to 8 bit before to get around this.
>
> Thanks,
>
> Michael
>
> _________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: [phone]  Cell: [phone]
>
> ------------------------------------------------------------