Re: non-destructive 2 channel merge question

Posted by Michael P Ellis on Mar 17, 2011; 6:45pm
URL: http://imagej.273.s1.nabble.com/non-destructive-2-channel-merge-question-tp3685343p3685349.html

Mike, hi

As already suggested, you are probably best off getting your 2 channel, time lapse image in the form of a hyperstack and using the composite display mode to display the merged the red and green channels.

I have written a plugin called "Composite Adjuster" that lets you conveniently adjust levels and colours whilst such a hyperstack is being animated. I am not sure if that would be of any interest to you but details here: http://www.dsuk.biz/DSUK/SmartCaptureLite.html

A YouTube clip of it in action here: http://www.youtube.com/embed/g0hoRLxBlWE?rel=0&hd=1

Regards -- Michael Ellis



On 17 Mar 2011, at 13:39, trshrthrth rdthjdrtjdrtj wrote:

> Hi folks,
>
> I am using imageJ v1.44n6 and I am working with a 2 channel (red/green) timelapse movie with Z-sections.
>
> I open this file with LOCI bioformats and use the “split channels” function. I then use the following to get a red/green overlay which i then save to .tif: image > color > merge channels > select the 2 channels > leave “create composite” blank > click ok.  However, I noticed that if prior to merging the 2 channels I have adjusted pixel intensities using the window/level tool, these are destructively carried over into my merged RGB file.  Is there a way to avoid this?
>
> I want to create an image where red and green are overlayed, but where the pixel intensities in both the red and green channels represent those of my raw data.  Really, the question is how does one create a red green overlay and save to .tif without destructively altering pixel intensity values.  Is it just a matter of avoiding using the window/level tool before merging??  I like to be as non-destructive as possible before getting my output .tif file into photoshop.
>
> Many thanks for your help.
>
> Mike