Re: non-destructive 2 channel merge question
Posted by
Glen MacDonald-2 on
Mar 17, 2011; 5:10pm
URL: http://imagej.273.s1.nabble.com/non-destructive-2-channel-merge-question-tp3685343p3685350.html
Dear Mike,
that is the nature of RGB conversion, the default assumption is you have selected the interesting portion of the source histogram. Use Adjust>B&C or >Color Balance on the Composite source images to either Set channels to 0-bit depth (eg 0 to 4095) and apply to all channels, or check the histograms and set to min/max for each channel. Then Image>Color>merge channels to RGB. alternatively, you can make your adjustments on merged Composite images then Image>Type>RGB.
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[hidden email]
On Mar 17, 2011, at 6:39 AM, trshrthrth rdthjdrtjdrtj wrote:
> Hi folks,
>
> I am using imageJ v1.44n6 and I am working with a 2 channel (red/green) timelapse movie with Z-sections.
>
> I open this file with LOCI bioformats and use the “split channels” function. I then use the following to get a red/green overlay which i then save to .tif: image > color > merge channels > select the 2 channels > leave “create composite” blank > click ok. However, I noticed that if prior to merging the 2 channels I have adjusted pixel intensities using the window/level tool, these are destructively carried over into my merged RGB file. Is there a way to avoid this?
>
> I want to create an image where red and green are overlayed, but where the pixel intensities in both the red and green channels represent those of my raw data. Really, the question is how does one create a red green overlay and save to .tif without destructively altering pixel intensity values. Is it just a matter of avoiding using the window/level tool before merging?? I like to be as non-destructive as possible before getting my output .tif file into photoshop.
>
> Many thanks for your help.
>
> Mike