JACoP

Posted by gauri on Mar 17, 2011; 3:27am
URL: http://imagej.273.s1.nabble.com/JACoP-tp3685369.html

Hello
I am using JACoP for colocalization , but it asks me for xy calibration and z calibration . i am not sure what values do i punch in there. it ask in nm. by default it shows 67nm for xy calibration and 200 for z calibration. i dont know what these values mean. i use confocal SP5 laser microscope and excitation wavelength of 488 and 561. Can somebody tell me what these xy calibration values should be and what do they mean.
Also it there is a column for x shift. what does that mean. i punched in some random numbers but it did not make any difference.

Gauri