Re: help with registration please

Posted by William Simpson on Mar 16, 2011; 8:29am
URL: http://imagej.273.s1.nabble.com/help-with-registration-please-tp3685383p3685385.html

Thanks very much Stephan and Carnë for your help.

Yes, Jodie and I are both working on this.
Yes, the objects being imaged are 3D.

3D registration I can see is a different approach, where you try to
reconstruct a 3D model of the object being imaged, and bring the 3D
elements into registration in 3D space. But should it not be possible
to use transformations in the plane, especially if the depth of the
elements is small compared to the height and width of the object being
imaged?

In this case, I would say that the depth is 1/5 of the height and width.

I am thinking that a better example to post here would be:
- image1
- image2 = image1 after being translated, sheared, and rotated
- show you the resulting registered stack.

I have a feeling that the result will not be great, simply because we
do not know how to optimise the registration routines. I am sure that
these routines are excellent! We are ignorant, and that is why we are
asking for expert advice here.

Thanks again for any help!

Cheers
Bill
On Tue, Mar 15, 2011 at 9:40 PM, Stephan Saalfeld <[hidden email]> wrote:

> Hi Bill-Jodie,
>
> to me it seems that you try to register two projections through a thick
> 3d volume in the projection plane.  That's not really possible without
> further knowledge of the 3d-structure or otherwise separable components
> of the signal.  If my impression is wrong, please correct me.
>
> Best,
> Stephan
>
>
>
>
> On Tue, 2011-03-15 at 17:27 +0000, William Simpson wrote:
>> Thanks for offering to help!
>> Here are the two original pics plus an animated .gif that is poorly registered.
>>
>> http://picpaste.com/AALtcc1-I1sU4DxK.png
>> http://picpaste.com/AALtcc2-eUb1IyDH.png
>> http://picpaste.com/AA_time_1_2_lt_cc_motion_bunwarp-Ij3FZv1v.gif
>>
>> Thanks for any help!
>> Bill
>