Watershed Segmentation

Posted by 765ooo on
URL: http://imagej.273.s1.nabble.com/Watershed-Segmentation-tp3685579.html

Hi
I am trying to segment stained nuclei that are in close contact/proximity.  However the watershed command segments the negative space between the nuclei instead of the nuclei.  

I have inverted the image, thresholded, played around with eroding the mask and more, but the segmentation of the nuclei is still faulty.