Posted by
Gabriel Landini on
Feb 12, 2011; 5:43pm
URL: http://imagej.273.s1.nabble.com/affordable-camera-suggestions-tp3685628p3685643.html
On Saturday 12 Feb 2011, <
[hidden email]> wrote:
> Normally people are using a professional camera normally with C-mount
> (that's why I mentioned C mount as a spec).
C mount a common thread in cameras and microscope adaptors. To attach a Nikon
SLR camera to a microscope you need an adaptor called F mount.
> DLSR camera's have color interpretation (Bayer) but most professional
> camera's for image analysis use it also
Some do, some don't
> So what will be the biggest difference? Noise?
I already mentioned this in a previous email. The Bayer mask over the sensor
has green red and blue pixels. To assign the 3 colours to a final "image
pixel", the Bayer mask is interpolated. So in reality, the resolution that the
manufacturer reports is not accurately the one in the acquired image. Have a
look at the wikipedia article on Bayer mask.
http://en.wikipedia.org/wiki/Bayer_maskCameras with the wheel can capture R , G and B for each pixel. The colour is
more accurate as it does not need to be the interpolated and so the resolution
is as specified byt number of pixels in the sensor. The problem is that as the
images are taken in sequence the objects should not move between shots. The
tunable filter cameras are quicker than the filter wheel ones (or at least the
one I have is quicker than the wheel ones I saw).
> So if I have to choose because of budget reasons between a low resolution
> professional camera or a high resolution DSLR camera, maybe the last is the
> best?
Which low resolution professional and which DSLR? The question does not make
much sense yet.
> Does anyone has experiences with using DSLR camera with image analysis?
Since you don't say what you are trying to achieve, here are some generic
suggestions.
Do not use jpegs. Use uncompressed (non-lossy) formats for your images (TIFF
or RAW (there is a plugin DC-RAW somewhere to read these).
Use a standardised illumination source so the images are comparable.
Use a fixed focal length in your shots so the magnification is also
comparable.
Add a colour calibration tablet to calibrate the colours and the
magnification, see:
http://imagejdocu.tudor.lu/doku.php?id=plugin:color:chart_white_balance:startUse the camera in Manual mode so you are in control of all the settings.
Use a tripod.
Cheers
Gabriel