Re: Watershed question

Posted by NatashaW on
URL: http://imagej.273.s1.nabble.com/Watershed-question-tp3685658p3685663.html

Karsten,

Many thanks for your explanation; you have exactly mentioned the most problems that I have found during my work!!
I’m really working to count/measuring some particles (foci) inside the nuclei. Daniel’s algorithm gives me good results when I work on the foci images (gray-level values).

But during this work, I found some problems:

1-When I smoothed the input image, the oversegmentation problem has been solved.. But watershed didn’t separate all the desired objects. I’m still looking for a better threshold value. Indeed, I didn’t find yet the best criteria to control the MIN and MAX values..

2-The detected particles are bigger than their real size. This may cause a problem during the measurement (like the mean and IntDen..) because the boundaries of the detected objects don’t match exactly their real size.. Maybe because of the chosen MIN value??

3-Is Daniel’s watershed algorithm an implementation of this article:
Vincent, Lee & Soille, Pierre, "Watersheds in digital spaces: An efficient algorithm based on immersion simulations", IEEE PAMI13 (6): 583-598
If not, could someone tell me which one? I want to understand how the algorithm works Please..

Thank you so much for your help