Re: Merging of Images

Posted by Katrien Remaut on Jan 31, 2011; 1:36pm
URL: http://imagej.273.s1.nabble.com/Merging-of-Images-tp3685871p3685874.html

That would be a very valuable tool for me, so I'm in favor of it!

Regards,

Katrien

-----Oorspronkelijk bericht-----
Van: ImageJ Interest Group [mailto:[hidden email]] Namens Crowell
Elizabeth
Verzonden: maandag 31 januari 2011 14:20
Aan: [hidden email]
Onderwerp: Re: Merging of Images

Hello,
On a related subject, while it's true that it may be impossible to
produce color figures that will be understandable by all types of color
blind people, it seems that the red/green/blue combination is one of the
least desirable.

Yet, the red/green/blue colors are the only colors proposed in the
Image/Color/Merge Channels tool.
Perhaps it might be worth considering allowing the use of more generally
discernable colors, like the widely used magenta/green combination or
blue/yellow combination noted below.  It would be nice, I think, to have
these other colors easily accessible in the Merge Channels tool.

What do the other users think?
Regards,
Elizabeth Crowell


Gabriel Landini a écrit :
> On Monday 31 Jan 2011 11:55:00 Phase GmbH wrote:
>  
>>  I am faced with the following problem:
>> A microscopic sample is labelled with 2 fluorescent dyes, one emitting
red
>> the other yellow. 2 Images are taken by a black/white camera, one for the
>> red label one, one for the yellow label. Every Image is colourized by a
>> applying the corresponding LUT.
>>
>> My question is know: What would be the best procedure to merge both
images?
>> The final image should show both labels in their correct colours.
>>    
>
> You can mix yellow and red but this will be a confusing orange, as the
hues
> are not that far away to produce a new distinct additive colou mix.
>
> I would rather put the "red" greyscale image (i.e. without LUT) in the
blue
> channel and the yellow signal (which you caputured in greyscale as well)
in
> both the red and green channels.
> This way ther "red" appears as "blue" and the "yellow" still as "yellow".
> Cololocalisation appears as white. This can be seen correctly by the vast
> majority of viewers, including all red-green colour blinds (e.g.
protanopes,
> deuteranopes, and anomalous prot- and deu- dichromats). Another advantage
is
> that they will perceive the colours with the correct names (unlike with
the

> green-magenta pairs that some use). Tritanopes however (a very small
> proportion of dichromatic viewers) might struggle to see the differences.
> There is no solution that fits all and preserves colour name perception.
>
> Note that the "correct colours" is somewhat misleading as there is no
> guarantee that the RGB LUT matches the emission spectrum of the dyes.
> I gave a talk on this subject at the last Luxembourg meeting.
>
> Regards
>
> Gabriel
>  

--

Elizabeth CROWELL

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