On Jan 31, 2011, at 8:53 AM, Winnok H. De Vos wrote:
> The ImageJ version of MacBioPhotonics offers a color merge function (as plugin) which allows to maintain the original LUT of images.
The Image>Color>Merge Channels command in ImageJ 1.44n or later maintains the original LUTs when "Create Composite" is checked in the dialog box.
> On 31 Jan 2011, at 14:36, Katrien Remaut wrote:
>
> That would be a very valuable tool for me, so I'm in favor of it!
>
> Regards,
>
> Katrien
>
> -----Oorspronkelijk bericht-----
> Van: ImageJ Interest Group [mailto:
[hidden email]] Namens Crowell
> Elizabeth
> Verzonden: maandag 31 januari 2011 14:20
> Aan:
[hidden email]
> Onderwerp: Re: Merging of Images
>
> Hello,
> On a related subject, while it's true that it may be impossible to
> produce color figures that will be understandable by all types of color
> blind people, it seems that the red/green/blue combination is one of the
> least desirable.
>
> Yet, the red/green/blue colors are the only colors proposed in the
> Image/Color/Merge Channels tool.
> Perhaps it might be worth considering allowing the use of more generally
> discernable colors, like the widely used magenta/green combination or
> blue/yellow combination noted below. It would be nice, I think, to have
> these other colors easily accessible in the Merge Channels tool.
>
> What do the other users think?
> Regards,
> Elizabeth Crowell
>
>
> Gabriel Landini a écrit :
>> On Monday 31 Jan 2011 11:55:00 Phase GmbH wrote:
>>
>>> I am faced with the following problem:
>>> A microscopic sample is labelled with 2 fluorescent dyes, one emitting
> red
>>> the other yellow. 2 Images are taken by a black/white camera, one for the
>>> red label one, one for the yellow label. Every Image is colourized by a
>>> applying the corresponding LUT.
>>>
>>> My question is know: What would be the best procedure to merge both
> images?
>>> The final image should show both labels in their correct colours.
>>>
>>
>> You can mix yellow and red but this will be a confusing orange, as the
> hues
>> are not that far away to produce a new distinct additive colou mix.
>>
>> I would rather put the "red" greyscale image (i.e. without LUT) in the
> blue
>> channel and the yellow signal (which you caputured in greyscale as well)
> in
>> both the red and green channels.
>> This way ther "red" appears as "blue" and the "yellow" still as "yellow".
>> Cololocalisation appears as white. This can be seen correctly by the vast
>> majority of viewers, including all red-green colour blinds (e.g.
> protanopes,
>> deuteranopes, and anomalous prot- and deu- dichromats). Another advantage
> is
>> that they will perceive the colours with the correct names (unlike with
> the
>> green-magenta pairs that some use). Tritanopes however (a very small
>> proportion of dichromatic viewers) might struggle to see the differences.
>> There is no solution that fits all and preserves colour name perception.
>>
>> Note that the "correct colours" is somewhat misleading as there is no
>> guarantee that the RGB LUT matches the emission spectrum of the dyes.
>> I gave a talk on this subject at the last Luxembourg meeting.
>>
>> Regards
>>
>> Gabriel
>>
>
>
> --
>
> Elizabeth CROWELL
>
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