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Re: Counting empty circles

Posted by schamber789 on Jan 07, 2011; 8:01pm
URL: http://imagej.273.s1.nabble.com/Counting-empty-circles-tp3686043p3686050.html

Hi Michael,

I wonder if Feature Finder plugin can be automated somehow. I know the website says that it does not process stacks, but is there any other way to process many images somehow with Feature Finder? I have thousands to process.

Thanks! Scott

On Jan 7, 2011, at 5:21 AM, Michael Schmid wrote:

> Hi Scott,
>
> you could try my 'Feature Finder' template matching plugin - I have put it online now:
>  http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:feature_finder:start
>
> It seems that the features of interest (trichomes) can appear differently, with or without the bright ring? So you might combine the results obtained with different templates (prototypes).
> And there are lots of parameters to try: different brightness/contrast of the template, different ROI size in the template, and all the parameters of the dialog box...
>
> Michael
> ________________________________________________________________
>
>> Hello,
>>
>> I have images (see below) that have many glandular trichomes (the round
>> shiny objects) which I would like to quantify. I simply want to know how
>> many of them there are.  It seems like I should be able to ask ImageJ to
>> count the number of these glandular trichomes, but I don't know how to do
>> this. I have tried many things already, but have hit a wall. The smaller
>> circles within each gland seem to be very consistent in size. I wonder if
>> this consistency in size can be used somehow. Thanks so much!
>>
>> Sincerely,
>>
>> Scott Chamberlain
>> Rice University, EEB Dept.
> <Feature_Finder_Test.jpg>