fluorescence intensity measurement

Posted by ylckyoto08 on Dec 21, 2010; 7:42am
URL: http://imagej.273.s1.nabble.com/fluorescence-intensity-measurement-tp3686123.html

Hi all,

I am trying with ImageJ to measure red fluorescent spots (against black background) in order to compare the intensity between several samples.  Could someone kindly tell me the detailed protocol how to do that?  I have searched through the ImageJ Manual but there isn`t specific instruction on fluorescence measurement.  Your help would be greatly appreciated.  Thanks!