Re: fluorescence intensity measurement

Posted by Rina on Dec 21, 2010; 12:27pm
URL: http://imagej.273.s1.nabble.com/fluorescence-intensity-measurement-tp3686123p3686124.html

Hello,
I used to that using this way:

duplicate image, to get (1) and (2)
select (2) => process => substract background
with (2): set threshold => apply
with (2): edit => selection => create selection
with (2): edit => selection => add to manager
go to (1) => click measure at the ROI manager window
“Mean” shows the gray level of the particels
with (2) => edit => selection => make inverse
delete number in ROI manager
with (1) => edit => selection => select none
go to ROI and click measure (shows the gray level of the background, so if you substract it from the first number you got your intensity)