Re: Calibration- pixels/um or um/pixel

Posted by Rasband, Wayne (NIH/NIMH) [E] on Dec 17, 2010; 4:50am
URL: http://imagej.273.s1.nabble.com/Calibration-pixels-um-or-um-pixel-tp3686165p3686167.html

On Dec 16, 2010, at 6:25 PM, David Knecht wrote:

> I was having trouble setting up calibrations in Micro-manager yesterday and finally figured out why.   Set Scale in ImageJ reads out in pixels/micron.  If you take those numbers and try to enter them into Micro-manager's calibration, it asks for the data as um/pixel.  The Micro-manager folks asked if it could be changed in ImageJ to conform to their setup.  I don't know if there is any "standard" way of doing this, but it would be nice if the two used the same units to avoid confusion. However, I am sure that if it is changed in ImageJ, lots of people might have their data altered.  Is there an easy way to resolve this?   Thanks- Dave

Use Image>Properties instead of Analyze>Set Scale. It displays the scale in microns/pixel. It also has the advantage of a convenient and easy to remember keyboard shortcut (shift-p).

-wayne