1. Create the one transformation file by registering the two reference
images. Then make symlinks to that transformation file for each image
you want to transform. Use Transform Virtual Stack Slices to transform
2. Create a script that does it. If I find some minutes I can help with
> Thanks Stephan,
> I have already played with these plugins but cannot get them
> to do what i want. The Transform Virtual Stack Slices plugin
> appears to require a separate transformation file for every
> image, what i want to do is apply a single transformation to
> multiple images. Is this possible with those plugins?
> James
>
> >
> >Check the Fiji plugins
> >
> >Register Virtual Stack Slices
> >
> >
http://pacific.mpi-> cbg.de/wiki/index.php/Register_Virtual_Stack_Slices
> >
> >and
> >
> >Transform Virtual Stack Slices
> >
> >
http://pacific.mpi-> cbg.de/wiki/index.php/Transform_Virtual_Stack_Slices
> >
> >they incorporate bUnwarpJ, feature based registration and
> allow you to
> >save the estimated transformation and apply it to other
> images later.
> >
> >Good luck,
> >Stephan
> >
> >
> >On Wed, 2010-12-15 at 11:24 +1100, James Burchfield wrote:
> >> I am looking for a simple way to measure the transformation
> >> required to achieve proper image registration between a set
> of
> >> reference images and then to apply this same transformation
> to a
> >> stack.
> >> I have been able to achieve fantastic results with bUnwarpJ
> on
> >> the reference image, but cannot work out how to apply this
> to
> >> other images.
> >> MultiStackReg works well on some data sets, but ideally i
> would
> >> like to apply exactly the same transformation to every
> image.
> >> Cheers,
> >> James