Re: Surface Area Confusion

Posted by Gabriel Landini on Dec 10, 2010; 12:15pm
URL: http://imagej.273.s1.nabble.com/Surface-Area-Confusion-tp3686211p3686214.html

On Thursday 09 December 2010 09:44:00 you wrote:
> A couple of people have requested a sample image, so here it is.
> This is the UV channel and the camera/software delineates certain spots in
> yellow that it deems UV damage.  We would like to determine the surface area
> of only these particular spots.

> What I have tried doing so far is converting the image to 8-bit, and then
> making it binary and then running "Analyze particles".

Using jpegs for imaging is not a good idea as they have lots of nasty
artifacts. That will prevent from segmenting the spots with certainty.

Also, it is not clear if the yellow areas were added by you or they are yellow
in the original image
It the yellow is originally there, you could try the colour threshold in
HSB mode, but the jpeg posted has lots of artifacts, so it is difficult to
know if that would work.

Cheers
Gabriel