Posted by
Cammer, Michael on
Dec 02, 2010; 1:36pm
URL: http://imagej.273.s1.nabble.com/Enhance-Contrast-with-16-bit-images-only-uses-256-bins-tp3686304p3686306.html
How about:
run("Median...", "radius=0.5");
makeOval(488, 595, 34, 37);
run("Measure");
run("Subtract...", "value=48");
run("Gamma...", "value=something you like
run("Brightness/Contrast...");
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Charles Brossollet
Sent: Thursday, December 02, 2010 4:46 AM
To:
[hidden email]
Subject: Enhance Contrast with 16 bit images only uses 256 bins
Hello,
I have problems using contrast enhancement on 16 bit gray-level images. My
images have 99% of pixel values in the 5% of true min-max range, so an
histogram with 256 bins will have huge pixel counts on a few bins. This
gives inappropriate contrast enhancement since it seems that ImageJ uses 256
bin histogram for this function.
I have an example image here:
http://dl.dropbox.com/u/2539804/TD1.tifGood LUT range for this image is about 5 to 250 levels, but the "enhance
contrast" with 0,35 gives 3 to 743, leading to a poorly contrasted display.
INMHO computing histogram on more bins should solve the problem.
Best regards,
Charles
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