http://imagej.273.s1.nabble.com/Problem-opening-Nikon-Elements-files-tp3686327p3686329.html
> The LOCI importer, which works fine on the Nikon Z and time series sequences, chokes on these files or opens them with the same weird colors as ImageJ directly.
Bio-Formats (4.2.1). You can see which version is installed in ImageJ by
If you see the problem with 4.2.1, then it is certainly something that we would fix in Bio-Formats; unfortunately, it is a bit difficult to fix without seeing
one of the problematic files. I have sent you a private email with instructions for sending files to us (the Bio-Formats developers), in case you are interested in providing an example file.
> More information on the problem:
>
> If there are only three channels in the image it opens as a 16 bit RGB stack. If there is one image, it opens as it should as a 16 bit single image. Any other numbers of channels gives the error "Unsupported SamplesPerPixel: #"
>
> Is there a way to open a file of any number into a simple 16 bit stack?
>
> Thanks!
>
> -Michael
>
> From: Cammer, Michael
> Sent: Tuesday, November 30, 2010 11:55 AM
> To:
[hidden email]
> Cc: 'Keith Gembala'; Liapis, Anastasia
> Subject: Problem opening Nikon ECammer, Michaellements files
>
> We are having problems opening NIS 3.0 files and hope that somebody has a solution.
>
> We are collecting four 16 bit images, three fluorescent probes and a brightfield image of fixed and stained cells. When we try to open them in ImageJ, they make a weird multicolor image. When we open in Photoshop, the first image in the series opens properly as a 16 bit grayscale, but the other images in the sequence are not accessible. ( see
http://www.flickr.com/photos/mcammer/5220769893/sizes/l/ for screen snap)
>
> There are two ways we have tried to save the files and each has a different odd result.
>
> One way is to save each individually as a TIFF. In this case, the file size is 2,236,838 bytes. (left image in screensnap)
>
> The second way is to have each sequence of four saved as the images are collected. In this case, we specify the format to be Nikon's, but the file is saved with a TIF extension. Renaming the tiff extension does not solve the problem. The file size is 2,322,552 bytes. (right image in screensnap)
>
> The LOCI importer, which works fine on the Nikon Z and time series sequences, chokes on these files or opens them with the same weird colors as ImageJ directly.
>
> Any help appreciated!
>
> Thank you.
>
>
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: (212) 263-3208 Cell: (914) 309-3270
>
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