Posted by
Vergara, Leoncio A. on
Nov 30, 2010; 10:48pm
URL: http://imagej.273.s1.nabble.com/Problem-opening-Nikon-Elements-files-tp3686327p3686330.html
I faced this issue a while ago, I no longer use NIS on a daily basis (changed work place), however I remember we got a macro for NIS (one of the Nikon reps installed it). It basically generated a sequence of monochrome 16-bit tiff files which could be read with image J with no problems and joined into a stack or multichannel image in the usual way. Hope this helps somehow.
Leoncio
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Cammer, Michael
Sent: Tuesday, November 30, 2010 11:22 AM
To:
[hidden email]
Subject: Re: Problem opening Nikon Elements files
More information on the problem:
If there are only three channels in the image it opens as a 16 bit RGB stack. If there is one image, it opens as it should as a 16 bit single image. Any other numbers of channels gives the error "Unsupported SamplesPerPixel: #"
Is there a way to open a file of any number into a simple 16 bit stack?
Thanks!
-Michael
From: Cammer, Michael
Sent: Tuesday, November 30, 2010 11:55 AM
To:
[hidden email]
Cc: 'Keith Gembala'; Liapis, Anastasia
Subject: Problem opening Nikon ECammer, Michaellements files
We are having problems opening NIS 3.0 files and hope that somebody has a solution.
We are collecting four 16 bit images, three fluorescent probes and a brightfield image of fixed and stained cells. When we try to open them in ImageJ, they make a weird multicolor image. When we open in Photoshop, the first image in the series opens properly as a 16 bit grayscale, but the other images in the sequence are not accessible. ( see
http://www.flickr.com/photos/mcammer/5220769893/sizes/l/ for screen snap)
There are two ways we have tried to save the files and each has a different odd result.
One way is to save each individually as a TIFF. In this case, the file size is 2,236,838 bytes. (left image in screensnap)
The second way is to have each sequence of four saved as the images are collected. In this case, we specify the format to be Nikon's, but the file is saved with a TIF extension. Renaming the tiff extension does not solve the problem. The file size is 2,322,552 bytes. (right image in screensnap)
The LOCI importer, which works fine on the Nikon Z and time series sequences, chokes on these files or opens them with the same weird colors as ImageJ directly.
Any help appreciated!
Thank you.
________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
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