normalization of fluorescence intensity quantification

Posted by jiami Guo on Nov 19, 2010; 2:11pm
URL: http://imagej.273.s1.nabble.com/normalization-of-fluorescence-intensity-quantification-tp3686376.html

Hi,

I am interested to show the fluorescence intensity difference of a lineage marker staining between stem cell and differentiating stem cell. I am thinking maybe I can just use nuclear staining as normalization to control for the focal plane differences and photobleaching. I want to measure area and intensity of each single cell and normalize each cell against their nuclear staining intensity. Can anybody tell me what's the best way to do it? Or is there better way to do quantify my staining? Any suggestion will be good! Thank you!

Jiami Guo