Re: Measuring signal intensity from an area enclosed between two ROIs

Posted by Cammer, Michael on Oct 29, 2010; 12:50pm
URL: http://imagej.273.s1.nabble.com/Measuring-signal-intensity-from-an-area-enclosed-between-two-ROIs-tp3686543p3686544.html

Before ImageJ had a band command, we would measure the big shape (intensity1 X area1) and then measure the small shape (intensity2 X area2) and then subtract the small shape's integrated intensity from the from the big shape's and divide by  the difference of the areas area to get the band mean.  

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Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

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From: ImageJ Interest Group [[hidden email]] On Behalf Of Avinash Kali [[hidden email]]
Sent: Friday, October 29, 2010 2:06 AM
To: [hidden email]
Subject: Measuring signal intensity from an area enclosed between two ROIs

I am trying to measure the signal intensity from a ring-like structure in my image.

I drew an ROI enclosing the inner circle of the ring and another ROI enclosing the outer circle of the ring. I have noticed that in ImageJ 1.43 we can select multiple ROIs from ROI manager at the same time. This makes the two ROIs appear at the same time on the image. But how can I measure the signal intensity from the area enclosed between these two ROIs?

I know that by calculating the signal intensities from the two ROIs and using some simple math we can calculate the signal intensity from the area of interest. But this method may not be entirely correct.

Thank You.

Avinash.

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