http://imagej.273.s1.nabble.com/opening-flex-format-images-tp3686580p3686585.html
encoding scheme. Unfortunately, the LWF decoder is commercial software,
can use to read that type of Flex file. This is possible due to the
> Hi Melissa,
> I was doing exactly what you reported (dragging the file onto the ImageJ
> toolbar). Once I used the Bio-Formats Importer, ..., I got a different
> error!!!
> "Sorry, there was a problem during import", and:
>
> " loci.formats.MissingLibraryException: The LuraWave decoding library,
> lwf_jsdk2.6.jar, is required to decode this file.
> Please make sure it is present in your classpath.
> at
> loci.formats.codec.LuraWaveCodec.initialize(LuraWaveCodec.java:153)
> at
> loci.formats.codec.LuraWaveCodec.decompress(LuraWaveCodec.java:85)
> at
> loci.formats.tiff.TiffCompression.decompress(TiffCompression.java:186)
> at loci.formats.tiff.TiffParser.getTile(TiffParser.java:584)
> at loci.formats.tiff.TiffParser.getSamples(TiffParser.java:709)
> at loci.formats.in.FlexReader.openBytes(FlexReader.java:208)
> at loci.formats.FormatReader.openBytes(FormatReader.java:739)
> at
> loci.formats.ChannelFiller.getLookupTableComponentCount(ChannelFiller.java:262)
> at loci.formats.ChannelFiller.setId(ChannelFiller.java:245)
> at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:480)
> at loci.formats.ChannelSeparator.setId(ChannelSeparator.java:238)
> at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:480)
> at loci.formats.DimensionSwapper.setId(DimensionSwapper.java:279)
> at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:480)
> at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:480)
> at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:480)
> at loci.plugins.in.ImportProcess.setId(ImportProcess.java:652)
> at
> loci.plugins.in.ImportProcess.initializeStack(ImportProcess.java:513)
> at loci.plugins.in.ImportProcess.execute(ImportProcess.java:142)
> at loci.plugins.in.Importer.showDialogs(Importer.java:125)
> at loci.plugins.in.Importer.run(Importer.java:77)
> at loci.plugins.LociImporter.run(LociImporter.java:77)
> at ij.IJ.runUserPlugIn(IJ.java:183)
> at ij.IJ.runPlugIn(IJ.java:150)
> at ij.Executer.runCommand(Executer.java:124)
> at ij.Executer.run(Executer.java:61)
> at java.lang.Thread.run(Thread.java:619)
> Caused by: Unable to instantiate service for interface
> loci.formats.services.LuraWaveService
> at
> loci.common.services.ServiceFactory.getInstance(ServiceFactory.java:152)
> at
> loci.formats.codec.LuraWaveCodec.initialize(LuraWaveCodec.java:150)
> ... 26 more
> Caused by: java.lang.reflect.InvocationTargetException
> at sun.reflect.NativeConstructorAccessorImpl.newInstance0(Native
> Method)
> at
> sun.reflect.NativeConstructorAccessorImpl.newInstance(NativeConstructorAccessorImpl.java:39)
> at
> sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:27)
> at java.lang.reflect.Constructor.newInstance(Constructor.java:513)
> at
> loci.common.services.ServiceFactory.getInstance(ServiceFactory.java:150)
> ... 27 more
> Caused by: java.lang.NoClassDefFoundError: com/luratech/lwf/lwfDecoder
> at
> loci.formats.services.LuraWaveServiceImpl.<init>(LuraWaveServiceImpl.java:77)
> ... 32 more
> Caused by: java.lang.ClassNotFoundException: com.luratech.lwf.lwfDecoder
> at java.net.URLClassLoader$1.run(URLClassLoader.java:200)
> at java.security.AccessController.doPrivileged(Native Method)
> at java.net.URLClassLoader.findClass(URLClassLoader.java:188)
> at java.lang.ClassLoader.loadClass(ClassLoader.java:307)
> at java.lang.ClassLoader.loadClass(ClassLoader.java:252)
> at java.lang.ClassLoader.loadClassInternal(ClassLoader.java:320)
> ... 33 more
>
> But it seems we are getting close to the solution!!!
> Any other idea? Maybe we've just found a bug that has to be fixed (I'll get
> in touch with the plugin's developer) or something's wrong with my Java.
> Thanks for your time!!!
>
> -----Mensaje original-----
> De: ImageJ Interest Group [mailto:
[hidden email]] En nombre de
> Melissa Linkert
> Enviado el: jueves, 21 de octubre de 2010 15:22
> Para:
[hidden email]
> Asunto: Re: opening .flex format images
>
> Hi Joaquim,
>
> > I do not know whether LOCI is unable to open my images or I'm not able to
> run it properly. When I try to open my flex file the following error occurs:
> "File is not supported format, a reader plugin is not available, or it was
> not found.
> > ¿can anyone help me?
>
> It sounds like you opened the file by selecting "File > Open" or by
> dragging the file onto the ImageJ toolbar. If you instead select
> "Plugin > LOCI > Bio-Formats Importer" and then choose the .flex file, then
> the file should be opened correctly.
>
> Regards,
> -Melissa
>
> On Thu, Oct 21, 2010 at 10:11:59AM +0200, Soriano.Joaquim wrote:
> > I've just tried LOCI and it didn´t worked (Thank you very much Melissa
> anyway),
> > The images I'm trying to open were captured by using a Perkin Elmer,
> Opera, High content screening system (a high through output confocal
> microscope). I do not know whether LOCI is unable to open my images or I'm
> not able to run it properly. When I try to open my flex file the following
> error occurs: "File is not supported format, a reader plugin is not
> available, or it was not found.
> > ¿can anyone help me?
> > I can send a file if anyone is interested/willing to help.
> > Thank you all!!!
> >
> > -----Mensaje original-----
> > De: ImageJ Interest Group [mailto:
[hidden email]] En nombre de
> Melissa Linkert
> > Enviado el: jueves, 21 de octubre de 2010 0:40
> > Para:
[hidden email]
> > Asunto: Re: opening .flex format images
> >
> > Hi Joaquim,
> >
> > You could use Bio-Formats:
> >
> >
http://loci.wisc.edu/bio-formats/imagej> >
> > which supports reading images in many file formats, including Flex.
> >
> > Regards,
> > -Melissa
> >
> > On Wed, Oct 20, 2010 at 10:07:36AM +0200, Soriano.Joaquim wrote:
> > > Hi there!
> > >
> > > I would like to open stacks of images that are saved in a .flex format.
> > >
> > > ¿is it possible?, ¿anyone knows how to do it?
> > >
> > > Thank you very much in advance.
> > >
> > >
> > >
> > > Joaquim Soriano Felipe.
> > >
> > > Confocal Microscopy Unit
> > >
> > > Spanish National Cancer Research Centre (CNIO)
> > >
> > > Melchor Fernández Almagro,3, E-28029 Madrid, Spain
> > >
> > > Phone + 34 917 328 000, exts.: 3161, 3162.
[hidden email] <mailto:
>
[hidden email]>
> > >
> > >
> > >
> > >
> > >
> > > **NOTA DE CONFIDENCIALIDAD** Este correo electrÿnico, y en su caso los
> ficheros adjuntos, pueden contener informaciÿn protegida para el uso
> exclusivo de su destinatario. Se prohÿbe la distribuciÿn, reproducciÿn o
> cualquier otro tipo de transmisiÿn por parte de otra persona que no sea el
> destinatario. Si usted recibe por error este correo, se ruega comunicarlo al
> remitente y borrar el mensaje recibido.
> > > **CONFIDENTIALITY NOTICE** This email communication and any attachments
> may contain confidential and privileged information for the sole use of the
> designated recipient named above. Distribution, reproduction or any other
> use of this transmission by any party other than the intended recipient is
> prohibited. If you are not the intended recipient please contact the sender
> and delete all copies.
> > >
> > >
> >
> > **NOTA DE CONFIDENCIALIDAD** Este correo electrónico, y en su caso los
> ficheros adjuntos, pueden contener información protegida para el uso
> exclusivo de su destinatario. Se prohíbe la distribución, reproducción o
> cualquier otro tipo de transmisión por parte de otra persona que no sea el
> destinatario. Si usted recibe por error este correo, se ruega comunicarlo al
> remitente y borrar el mensaje recibido.
> > **CONFIDENTIALITY NOTICE** This email communication and any attachments
> may contain confidential and privileged information for the sole use of the
> designated recipient named above. Distribution, reproduction or any other
> use of this transmission by any party other than the intended recipient is
> prohibited. If you are not the intended recipient please contact the sender
> and delete all copies.
>
> **NOTA DE CONFIDENCIALIDAD** Este correo electrónico, y en su caso los
> ficheros adjuntos, pueden contener información protegida para el uso
> exclusivo de su destinatario. Se prohíbe la distribución, reproducción o
> cualquier otro tipo de transmisión por parte de otra persona que no sea el
> destinatario. Si usted recibe por error este correo, se ruega comunicarlo al
> remitente y borrar el mensaje recibido.
> **CONFIDENTIALITY NOTICE** This email communication and any attachments may
> contain confidential and privileged information for the sole use of the
> designated recipient named above. Distribution, reproduction or any other
> use of this transmission by any party other than the intended recipient is
> prohibited. If you are not the intended recipient please contact the sender
> and delete all copies.
>