Re: Raw versus calibrated integrated density

Posted by IJperson on Oct 19, 2010; 7:53am
URL: http://imagej.273.s1.nabble.com/Raw-versus-calibrated-integrated-density-tp3686613p3686617.html

Michael,

Many thanks. Its the units of the integrated density that has been confusing me.  But it seems it has unit of <spatial unit>^2

So in your new example, with the um calibration the result is 10000 um^2, and with the raw value it is 2500 pixel^2
And with one pixel equalling 4um^2 (2x2) I can see how you can convert between 2500 pixel^2 and 10000 um^2

I still don't quite understand how the units of the integrated density is solely a spatial area, but there you go, at least I understand the maths now! Thanks to everyone for pointing out this IS confusing, and its not just me!

Andrew