Posted by
Michael Schmid on
Oct 19, 2010; 10:08am
URL: http://imagej.273.s1.nabble.com/Raw-versus-calibrated-integrated-density-tp3686613p3686618.html
Hi Andrew,
in the general case, the unit of integrated density is <grayscale
unit>*<spatial unit>^2.
You get <spatial unit>^2 only if you don't have a grayscale unit.
Michael
__________________________________________________________________________
On Tue, October 19, 2010 09:53, IJperson wrote:
> Michael,
>
> Many thanks. Its the units of the integrated density that has been
> confusing
> me. But it seems it has unit of <spatial unit>^2
>
> So in your new example, with the um calibration the result is 10000 um^2,
> and with the raw value it is 2500 pixel^2
> And with one pixel equalling 4um^2 (2x2) I can see how you can convert
> between 2500 pixel^2 and 10000 um^2
>
> I still don't quite understand how the units of the integrated density is
> solely a spatial area, but there you go, at least I understand the maths
> now! Thanks to everyone for pointing out this IS confusing, and its not
> just
> me!
>
> Andrew
> --
> View this message in context:
>
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