Re: Raw versus calibrated integrated density

Posted by Gabriel Landini on Oct 18, 2010; 3:29pm
URL: http://imagej.273.s1.nabble.com/Raw-versus-calibrated-integrated-density-tp3686613p3686621.html

On Monday 18 Oct 2010  16:21:12 IJperson wrote:
> Many thanks for the reply.
> To clarify, I am not referring to greyscale calibration. Lets assume I am
> just interested in the intensity of the pixels as they appear, with no
> extra scaling.  My confusion lies with different integrated intensities
> (IntDen and RawIntDen) over spatial calibration.
 
This has been discussed many times in this mailing list.
I suggest that you search for "Integrated density" and see that the values do
change if you take into consideration spatial calibration in the computation,
but you do not gain any more information.

I personally think it is more intuitive to calculate the integrated density in
pixels (i.e. samples, not calibrated units) and provide the interpixel spacing
for reference of the resolution of the measurement. The measurement is then
just the sum of the greyscale values of the blob.

Regards

Gabriel