Posted by
Glen MacDonald-2 on
Oct 12, 2010; 3:45pm
URL: http://imagej.273.s1.nabble.com/Getting-the-scale-tp3686642p3686645.html
Well... to begin, how were the images collected, and in what file format were they saved? Have the images been converted from the original format into something else such as TIFF? Most acquisition software will record the lateral and axial dimensions into the file. what do you see in the Image>Properties or Image>Get Info... windows?
Opening the files with the LOCI plugin may present spatial information not accessible by the standard ImageJ opener.
Did you collect them, do you have access to the instrument? If you know the lens, zoom, box size (if confocal), you could place a ruler or stage micrometer on the microscope and collect an image. then measure the number of pixels per mm with imageJ. Details are in the documentation for Analyze>Set Scale. Alternatively, just setting up the instrument to match the collection settings may display the lateral scale.
If the axial scale cannot be extracted from the metadata, then the best you can do is estimate by comparison with some images collected while actually writing down the capture parameters.
Look at the Imagej documentation
http://imagej.nih.gov/ij/docs/Good luck,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[hidden email]
On Oct 12, 2010, at 4:17 AM, smfmazz wrote:
> Hi All,
> Excuse me for my english. I take advantage of this topic. I want get the
> distance inter-pixel in mm... How can i make? Can i use the method get
> calibration and the method get unit?
> If i have a stack, how i get the distance inter-slice (mm) ?
> Thanks a lot
> --
> View this message in context:
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