http://imagej.273.s1.nabble.com/difference-between-tiff-types-tp3686715p3686719.html
> 2010/10/6 Gabriel Landini <
[hidden email]>
>
>> On Oct 5, 2010, at 1:40 PM, IMAGEJ Алексей Малышко wrote :
>>> i have made some brightness adjustments in ImageJ and saved image (in
>>> 16-bit grayscale). but when i opened it in irfanview (and other image
>>> viewers) i saw that image didn't change (like a switched off layer in
>>> photoshop). then i reopened with imagej and saw changed image. then i
>>> converted it in imagej to rgb and only after that changes became visible
>>> in imageviewers.
>>
>> Does that still happen if you press the Apply button in the
>> Brightness/Contrast dialog after adjusting the contrast and before saving?
>>
>> Cheers
>>
>> Gabriel
>>
> YES! the "Apply" button, that's the answer. but... now i know a lot about
> images )
> But how it does save in such a way, that after reopening in ImageJ (IJ) the
> image IJ "remembers" how to display it and other image viewers don't?
ImageJ saves the min and max displayed pixel values in the ImageDescription tag. You can see the contents of this tag by opening a tiff file with ImageJ in debug mode, which is enabled in the Edit>Options>Misc dialog box.