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Re: Olympus .vsi file format?

Posted by Melissa Linkert-2 on Sep 28, 2010; 6:19pm
URL: http://imagej.273.s1.nabble.com/Olympus-vsi-file-format-tp3686779p3686783.html

Hi Kenneth,

As Dan mentioned, support for the VSI files can definitely be added to
Bio-Formats (http://loci.wisc.edu/bio-formats).  However, you do not
need to do this work yourself - if you can provide us (the Bio-Formats
developers) with a few example VSI files, we will happily work toward
adding support for these files in the near future.  I will send you our SFTP
server details off-list, in case you are interested in sending a few
files.

It would, of course, be wonderful to have a specification or some other
documentation, but for the majority of file formats that we have seen
this kind of information is simply not available.

Regards,
-Melissa

On Mon, Sep 27, 2010 at 09:36:59PM -0500, Kenneth Sloan wrote:

> I also suspect that it's a closed format - I was hoping that someone would disabuse me of that idea.
> Right now, I'm one person removed from the local Olympus rep, so I haven't had a chance to ask the
> right questions.  The impression I get from various literature
> is that the format might be closed - but sometimes it's possible to get documentation if you ask nicely.
> Sometimes, if you ask 17 times, they actually write the documentation...
>
> I've found a couple of systems that claim to read VSI files (anyone heard of mScope?)
>
> The system does indeed export all sorts of formats which I can deal with - but (warning: I have not
> actually touched the system, yet) a colleague reports that the TIFF files he's getting are not as good
> as the VSI files displayed using their software.  I'm hoping that he simply hasn't read the entire manual, yet.
>
> If we can import and export, I can build a workaround - but I have a strong preference for getting
> the RAW data out of any instrument ASAP and processing it with code that I have written.   Sometimes "lossless" is
> too lossy.
>
> Ah...here's the spec sheet - we are talking about the cellSens imaging software.  Spec sheet says it can read and write:
> VSI, JPEG, JPEG2000, TIFF, BMP, AVI, PNG.  Read-only: GIFF, PSD, TIFF (DP-BSW, FSX100,MetaMorph), OIR/OIB(Fluoview format)...
>
> I understand that we have actually managed to get the software to give us a PSD file.  Hmmm...I guess that's what they mean by "read-only".  These images are excellent, and we're using Photoshop to convert to beautiful TIFF.  I'm told that the TIFF files created directly are deficient (low resolution?  not deep enough?  don't know).
>
> So...cellSens->PSD->Photoshop->TIFF appears to work.  
>
> cellSens->TIFF *should* work - but something is not understood at the moment.
>
> I would *like* to directly read the VSI files - but I suspect that will be more trouble than it will be worth.
>
> My collaborator would dearly love to IMPORT a PSD file (after marking up the image using Photoshop).  I doubt that will
> be possible.
>
> We don't want to build a commercial product, and we are purchasing the software.  Is is too much to ask for documentation of
> the file formats created by the software?
>
> Closed formats are the bane of my existence!  Reverse-engineering them is a royal PITA.
>
> Oh well...I tried.
>
>
> On another topic: will there be anyone in Luxembourg interested in OCT imaging/segmentation?  If so, an informal lunch or dinner "session" might be interesting.
>
>
> On Sep 27, 2010, at 04:54 , Gabriel Landini wrote:
>
> > On Monday 27 Sep 2010  10:47:58 Daniel James White wrote:
> >
> >> I never heard of this file format before.
> >> What  olympus imaging system is it from ?
> >
> > Hi
> > It is the 'virtual slide' format used in the Olympus slide scanners (dotSlide
> > and other models).
> >
> > I have the suspicion (but hope not) that this might be a closed format.
> > If you have the Olympus scanner system accessible, then one should be able to
> > re-save the data in tif tiles (non-lossy) or jpeg (lossy).
> >
> > Cheers
> >
> > Gabriel
>
> --
> Kenneth Sloan
> [hidden email]