Re: Multithreshold question

Posted by Mario Faretta on Sep 20, 2010; 12:33pm
URL: http://imagej.273.s1.nabble.com/Multithreshold-question-tp3686868p3686869.html

  Il 9/20/2010 2:14 PM, Hanna Sas Nowosielska ha scritto:

> Dear Image J users,
>
> I'm new to image j and for last couple of days I'm struggling with a problem
> to with I can't find a solution. I would like to group pixels on my image to
> few intensity-based classes (based on the histogram) and then assign to each
> of the classes different color in the LUT. As a result I wont to get
> something like a thermal-map (but not gradient map) showing significant
> differences between fluorescence intensity. Unfortunately I can't find a
> solution how revalue pixels on a picture so that I get 5-6 classes.
>
> I will be very grateful for any suggestions,
> Best Regards,
> Hanna
>

Maybe the k-means clustering plugin
(http://ij-plugins.sourceforge.net/plugins/clustering/index.html) or the
multi-otsu threshold
(http://rsb.info.nih.gov/ij/plugins/multi-otsu-threshold.html) can help you
Mario

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Mario Faretta
Department of Experimental Oncology
European Institute of Oncology
c/o IFOM-IEO Campus for Oncogenomics
via Adamello 16 20139 Milan Italy
Phone: ++39-0294375027
email: [hidden email]
http://www.ifom-ieo-campus.it