Re: Removing uneven illuminaiton

Posted by Johannes-P. Koch on Aug 11, 2010; 9:43am
URL: http://imagej.273.s1.nabble.com/Removing-uneven-illuminaiton-tp3687023p3687024.html

Donny,

either you have a plain image, just with your background, then you can
perform an a priori shading correction, otherwise you can use an a
posteriori corrector...

check this out:
http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_background_illumination_in_brightfield_microscopy 



BTW: I have the mentioned a posteriori shading corrector from the
University of Reims - the Professor there is now retired so he took his
page off the web, unfortunately all the plugins are gone as well; for me
it works extremely well. if you want to test this particular corrector,
let me know and I will mail you the jar file.

Best,
Johannes


Am 11.08.2010 11:33, schrieb Donny George:

> Hello
>
> I have been trying to look for methods to remove uneven illumination form
> images. I did try  t      he built-in "Subtract Background" command (Process
> ->
> Subtract Background) and from a thread on i      magej  the plugin
> "Background Correction"  (http://rsb.info.nih.gov/ij/plugins/background.html
> ). But none of them provided useful to me. The images are taken from the
> production line with a camera and the flash system causes the uneven
> illumination. Can someone who has already tried making images homogenous
> help me with my task
>
> thank you
>
>    

--
Mag. Johannes-P. KOCH
Department of Biochemistry and Cell Biology
MFPL, University of Vienna
Dr. Bohrgasse 9/5
A-1030 Vienna
Austria

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