Login  Register

Re: Removing uneven illuminaiton

Posted by Marko Usaj on Aug 12, 2010; 10:41am
URL: http://imagej.273.s1.nabble.com/Removing-uneven-illuminaiton-tp3687023p3687033.html

If it possible, i would also like this the posteriori shading correction jar file. Thank you!

Regards,
Marko

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Lim Soon Yew
Sent: Thursday, August 12, 2010 4:01 AM
To: [hidden email]
Subject: Re: Removing uneven illuminaiton

Hi Johannes,

I also need the posteriori shading corrector jar file as well. Can you please e-mail it to me? Thank you.

Best Regards,
John
________________________________________
From: ImageJ Interest Group [[hidden email]] On Behalf Of Amrish Chawla [[hidden email]]
Sent: Wednesday, August 11, 2010 10:47 PM
To: [hidden email]
Subject: Re: Removing uneven illuminaiton

hi Johannes,

I need the posteriori shading corrector jar file as well. Can you email it
to me

Thanks,
Amrish
-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Johannes-P. Koch
Sent: Wednesday, August 11, 2010 5:43 AM
To: [hidden email]
Subject: Re: Removing uneven illuminaiton

Donny,

either you have a plain image, just with your background, then you can
perform an a priori shading correction, otherwise you can use an a
posteriori corrector...

check this out:
http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_backgrou
nd_illumination_in_brightfield_microscopy



BTW: I have the mentioned a posteriori shading corrector from the
University of Reims - the Professor there is now retired so he took his
page off the web, unfortunately all the plugins are gone as well; for me
it works extremely well. if you want to test this particular corrector,
let me know and I will mail you the jar file.

Best,
Johannes


Am 11.08.2010 11:33, schrieb Donny George:
> Hello
>
> I have been trying to look for methods to remove uneven illumination form
> images. I did try  t      he built-in "Subtract Background" command
(Process
> ->
> Subtract Background) and from a thread on i      magej  the plugin
> "Background Correction"
(http://rsb.info.nih.gov/ij/plugins/background.html
> ). But none of them provided useful to me. The images are taken from the
> production line with a camera and the flash system causes the uneven
> illumination. Can someone who has already tried making images homogenous
> help me with my task
>
> thank you
>
>

--
Mag. Johannes-P. KOCH
Department of Biochemistry and Cell Biology
MFPL, University of Vienna
Dr. Bohrgasse 9/5
A-1030 Vienna
Austria

phone: 0043 1 4277 52809
fax: 0043 1 4277 9528

mail to: [hidden email]