Re: hacking help, please

Posted by dscho on Sep 01, 2010; 10:13am
URL: http://imagej.273.s1.nabble.com/hacking-help-please-tp3687063p3687064.html

Hi,

On Tue, 31 Aug 2010, Kenneth Sloan wrote:

> I would appreciate pointers, clues, and hints on how to do the following:
>
> a) outline a large, blob-like region in an image
> b) within that region, outline MANY small included regions
> c) report:
> for the large blob: area
>         for EACH small included blob:
> centroid
> area
> integrated intensity
> d) subtract all of the included blobs from the large blob, and then report:
> remaining area
> integrated intensity over that area

My suggestion:

a) mark the big blob with the freehand ROI tool

b) hold down the Alt key and subtract the many small blobs inside (they
   must not overlap!)

c) add the ROI to the ROI Manager and use More>>Split to get at the
   individual blobs

   mark all ROIs in the ROI manager and Measure (after activating all the
   relevant things in Analyze>Set Measurements...)

The first row in the results table will contain the results for the
"remaining area", the second row for the overall area, and then come the
results for the individual blobs.

No need for additional programming, I guess ;-)

Ciao,
Johannes