http://imagej.273.s1.nabble.com/hacking-help-please-tp3687063p3687066.html
> I would appreciate pointers, clues, and hints on how to do the following:
>
> a) outline a large, blob-like region in an image
> b) within that region, outline MANY small included regions
> c) report:
> for the large blob: area
> for EACH small included blob:
> centroid
> area
> integrated intensity
> d) subtract all of the included blobs from the large blob, and then report:
> remaining area
> integrated intensity over that area
>
> I can see most of the pieces by pointing and clicking and reading the
> manual - but it would really help
> to see something close to an actual implementation.
>
> My immediate stumbling blocks are:
> * displaying the enclosing blob boundary AND the boundaries of all
> the smaller blobs
> (my problem is that I can only see the current blob - which then
> disappears when I start on the next one)
> * details on managing the results
>
> I'm probably more comfortable writing Java code - but I'd be happy to try
> to do this as a macro.
>
> I'll probably want to use a polygon for the large blob boundary - and fit
> ellipses to the smaller blobs.
>
> If you care - what I'm measuring are cone inner segments in human retina
> and mitochondria. (actually, someone else will sit
> at the image and count them - I'm the GUI guy).
>
> To calibrate - I've been creating systems to help people make these sorts
> of measurements for some 30+ years, and am comfortable writing fairly
> complicated pieces of Java code - what I need help on are the nitty gritty
> details.
>
> (perhaps all I need are pointers of the form: "check out tutorial #'s 3, 5,
> 11, and 43")
>
> Thanks very much - all clues gratefully accepted.
>
> With luck, I can demo the resulting system, and show off some results, in
> Luxembourg.
>
> --
> Kenneth Sloan
>
[hidden email]
>