http://imagej.273.s1.nabble.com/hacking-help-please-tp3687063p3687067.html
> Measurements ..."? This should get a,b, and c.
>
> On Tue, Aug 31, 2010 at 8:27 PM, Kenneth Sloan <
[hidden email]>wrote:
>
>> I would appreciate pointers, clues, and hints on how to do the following:
>>
>> a) outline a large, blob-like region in an image
>> b) within that region, outline MANY small included regions
>> c) report:
>> for the large blob: area
>> for EACH small included blob:
>> centroid
>> area
>> integrated intensity
>> d) subtract all of the included blobs from the large blob, and then report:
>> remaining area
>> integrated intensity over that area
>>
>> I can see most of the pieces by pointing and clicking and reading the
>> manual - but it would really help
>> to see something close to an actual implementation.
>>
>> My immediate stumbling blocks are:
>> * displaying the enclosing blob boundary AND the boundaries of all
>> the smaller blobs
>> (my problem is that I can only see the current blob - which then
>> disappears when I start on the next one)
>> * details on managing the results
>>
>> I'm probably more comfortable writing Java code - but I'd be happy to try
>> to do this as a macro.
>>
>> I'll probably want to use a polygon for the large blob boundary - and fit
>> ellipses to the smaller blobs.
>>
>> If you care - what I'm measuring are cone inner segments in human retina
>> and mitochondria. (actually, someone else will sit
>> at the image and count them - I'm the GUI guy).
>>
>> To calibrate - I've been creating systems to help people make these sorts
>> of measurements for some 30+ years, and am comfortable writing fairly
>> complicated pieces of Java code - what I need help on are the nitty gritty
>> details.
>>
>> (perhaps all I need are pointers of the form: "check out tutorial #'s 3, 5,
>> 11, and 43")
>>
>> Thanks very much - all clues gratefully accepted.
>>
>> With luck, I can demo the resulting system, and show off some results, in
>> Luxembourg.
>>
>> --
>> Kenneth Sloan
>>
[hidden email]
>>