Help with Fluoview imports (UCSD plugin, LOCI tools)

Posted by jesse_brann on Aug 12, 2010; 2:59am
URL: http://imagej.273.s1.nabble.com/Help-with-Fluoview-imports-UCSD-plugin-LOCI-tools-tp3687268.html

Hello,

I'm having a problem with ImageJ after the most recent Java update.  I'm using the latest version of ImageJ (1.43) with the newest Java version (1.6.0_10).

This problem happens on both our lab Macs (current operating system) and on a Windows computer with Windows XP Professional Version 2002 Service Pack 3.

I can no longer open Fluoview images with the UCSD confocal microscopy plugin.  I am attempting to open my confocal images (3-color z series, no time component).  I've tried opening them with all of the various ImageJ File-Open menu options. Nothing works.  

I next tried installing the LOCI Bioformats plugin.  I managed to get the stack to open as a hyperstack, but by using the colorized color mode and split channels.  However, I would like to see what the UCSD plugin used to do - open the Fluoview stack as a merged, three-color image that I could navigate by focal plane.  I am attempting to quantify co-localization, so the lack of the merged color is a problem.  

Thank you for any help you can provide - even simple instructions on how to properly open the Fluoview .tifs would be helpful!

-Jessica

Do you  know how I can fix this?