Re: SCALE

Posted by Colin Rickman-3 on Aug 08, 2010; 8:50am
URL: http://imagej.273.s1.nabble.com/SCALE-tp3687327p3687328.html

Hi

You need to know two bits of information. The physical size of a pixel on your CCD in your camera and the total magnification between your sample and the detector. Divide the physical pixel size by the magnification and the answer is the size of one of your pixels in the displayed image.

Colin

Dr Colin Rickman
Department of Chemistry (WP 2.03)
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)
Tel: [phone]
Fax: [phone]
http://www.eps.hw.ac.uk/departments/chemistry/cr.htm



On 8 Aug 2010, at 06:42, "Hughes, Shawna" <[hidden email]> wrote:

> Hello,
>
> I am new to the ImageJ software and I have a question, I am working with dough and analyzing air bubbles of the dough with a light microscope. The light microscope does not have a scale and I was wondering how do I get a so that I can start accumulating the results. I will be measuring the area, length, and width.
>
> I really do need help.
> Thank you in advance
>
> Shawna Hughes
>
> Shawna Hughes
> Graduate Student, Food Science
> NSF Bridge to Doctorate Fellow
> Advisor: Dr. Rayas-Duarte
> Oklahoma State University
> Robert M. Kerr Food & Agricultural Products Center
> Stillwater, OK 74078