Login  Register

Re: convert video stream in stack

Posted by dscho on Aug 03, 2010; 8:34am
URL: http://imagej.273.s1.nabble.com/multichannel-3D-viewing-problem-with-Fluoview500-tiffs-tp3687386p3687394.html

On Mon, 2 Aug 2010, Felipe Franco wrote:

>           Guys,

            Girl,

>           I m working in a molecular imaging project and we need to
>           measure the fluorescence of organs in diferents intervals, not
>           in a specific time. I just cannot find any software that is
>           able to take an video stream ( working in set intervals ) and
>           transforme it in a stack .tiff. Do you guys have some
>           sugestion? Do you guys think that if i convert my .vob file (
>           dvd video ) in quick time and then into a stack will lose some
>           quality?

There are a number of tools, the easiest method on Linux is to use mplayer
with the "-vo png" option to populate a directory of .png files which you
then read back via File>Import>Image Sequence...

However, guessing (I cannot know because you neglected to let us know that
pretty important piece of information) that you are rather a Windows guy,
you could use VirtualDub:

http://www.virtualdub.org/blog/pivot/entry.php?id=34

By the way, I fail to see the reason why your mail is part of the
"multichannel 3D viewing problem with Fluoview500 tiffs + orthosections"
thread:

http://thread.gmane.org/gmane.comp.java.imagej/18923/focus=19032

Hth,
Johannes