Settings for Particle Analysis

Posted by FloKun on Jul 29, 2010; 3:19pm
URL: http://imagej.273.s1.nabble.com/Settings-for-Particle-Analysis-tp3687429.html

Hello

With the help of microscope pictures of my o/w emulsion I am trying to get a PSD. My problem is that the particles nearly have the same colour as the background, the PSD is very broad and the particles are very close together (picture 1). To enhance the contrast I coloured my Oil with Sudan III (picture 2). It helps but I would prefer to relinquish Sudan III. Furthermore I dilute the emulsion to get fewer particles on each picture (3).

Nevertheless ImageJ does not do what I want. The particles are easy to see but I do not know how to tell that ImageJ. So if anybody knows some adjustments or tricks how to get my particles analysed, please let me know.

Thank you very much and greetings from Hamburg/Germany