Re: Need trouble shooting advice for deconvolution

Posted by Piotr Wendykier-2 on Jun 15, 2010; 12:09am
URL: http://imagej.273.s1.nabble.com/Need-trouble-shooting-advice-for-deconvolution-tp3687551p3687558.html

Dave,

As Dan pointed out, you should open zvi files using LOCI bio-formats importer.
You don't need to manually convert the images into 32-bit floating
point type - they will be converted inside Parallel Iterative
Deconvolution plugin, which uses floating point numbers to do all the
processing.

Regards,

Piotr

On Mon, Jun 14, 2010 at 5:15 PM, daschneider9 <[hidden email]> wrote:

> Piotr,
>
> Thank you for your suggesting. The original zvi file stores each channel as
> 16 bit grey scale. Am I correct that I should not rescale these to 32 bit
> befoer deconvolution?
>
> Dave
> --
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>