RE: Need trouble shooting advice for deconvolution

Posted by daschneider9 on Jun 15, 2010; 12:16am
URL: http://imagej.273.s1.nabble.com/Need-trouble-shooting-advice-for-deconvolution-tp3687551p3687559.html

Thank you Piotr.

 

I can do that but get the following exception list:

 

java.lang.NullPointerException

                at ij.gui.ImageWindow.close(ImageWindow.java:371)

                at ij.gui.StackWindow.close(StackWindow.java:139)

                at ij.ImagePlus.close(ImagePlus.java:331)

                at loci.plugins.importer.Importer.displayStack(Importer.java:691)

                at loci.plugins.importer.Importer.showStack(Importer.java:559)

                at loci.plugins.importer.Importer.run(Importer.java:401)

                at loci.plugins.LociImporter.run(LociImporter.java:77)

                at ij.IJ.runUserPlugIn(IJ.java:189)

                at ij.IJ.runPlugIn(IJ.java:155)

                at ij.Executer.runCommand(Executer.java:147)

                at ij.Executer.run(Executer.java:78)

                at java.lang.Thread.run(Unknown Source)

 

I don’t understand what this is telling me but the imported channel images appear as expected otherwise.

 

I appreciate your advice and look forward to becoming more proficient at this, even if not ever an expert.

 

Kind regards,

Dave

 

From: Piotr Wendykier-2 [via ImageJ] [mailto:[hidden email]]
Sent: Monday, June 14, 2010 5:12 PM
To: Schneider, David A.
Subject: Re: Need trouble shooting advice for deconvolution

 

Dave,

As Dan pointed out, you should open zvi files using LOCI bio-formats importer.
You don't need to manually convert the images into 32-bit floating
point type - they will be converted inside Parallel Iterative
Deconvolution plugin, which uses floating point numbers to do all the
processing.

Regards,

Piotr

On Mon, Jun 14, 2010 at 5:15 PM, daschneider9 <[hidden email]> wrote:

> Piotr,
>
> Thank you for your suggesting. The original zvi file stores each channel as
> 16 bit grey scale. Am I correct that I should not rescale these to 32 bit
> befoer deconvolution?
>
> Dave
> --
> View this message in context: http://imagej.588099.n2.nabble.com/Need-trouble-shooting-advice-for-deconvolution-tp5172219p5179557.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>

 

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