Re: Automatic Cell Counting

Posted by Barron, Francie on Jul 01, 2010; 4:51am
URL: http://imagej.273.s1.nabble.com/Re-Automatic-Cell-Counting-tp3687675.html

Hi,

I'm new to this program, so hope to receive some help.

I have paraffin sections through a mouse E11.5 mandibular arch that I have stained for proliferation and cell death.  I wanted to count the total number of cells (via DAPI) and compare that to cell proliferation positive and cell death positive cells in both control and mutant (I have them stored as 16-bit RGB as well as 8-bit).  As of now, I've been using the manual cell counter plug-in through Fuji, but would like a way to specify an ROI and automate this process.  I have downloaded and tried the UCSB ITCN plugin for automation, but it was not able to count cells closely packed together despite a low threshold.  If anyone can suggest a way to automate this (a new plugin or one contained into ImageJ), or help with the plugins that I have that would be great.

Thanks so much!!
~Francie



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Francie Barron (Hyndman), Graduate Student
Cells Biology, Stem Cells, and Development Program
Dr. David Clouthier lab, Craniofacial Biology

For Correspondence:
University of Colorado, Health Sciences Center
Department of Craniofacial Biology
Mailstop 8120
P.O. Box 6511
Aurora, CO 80045

For Deliveries:
University of Colorado Denver Health Sciences Center
Department of Craniofacial Biology
Building RC1 South, Room 11400B
12801 East 17th Avenue
Aurora, Co 80010

Lab: 303-724-4566
Fax: [phone]

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