Posted by
Daniel James White on
Jul 02, 2010; 9:50am
URL: http://imagej.273.s1.nabble.com/Re-Automatic-Cell-Counting-tp3687675p3687677.html
Hi Francie,
send me a sample image and i will make some suggestions....touching nuclei are often very hard to get.
A watershed method might work, see watershed here.
http://rsbweb.nih.gov/ij/docs/menus/process.html#binarycheers
Dan
On Jul 2, 2010, at 6:00 AM, IMAGEJ automatic digest system wrote:
>
> Date: Wed, 30 Jun 2010 22:51:15 -0600
> From: "Barron, Francie" <
[hidden email]>
> Subject: Re: Automatic Cell Counting
>
> Hi,
>
> I'm new to this program, so hope to receive some help.
>
> I have paraffin sections through a mouse E11.5 mandibular arch that I have =
> stained for proliferation and cell death. I wanted to count the total numb=
> er of cells (via DAPI) and compare that to cell proliferation positive and =
> cell death positive cells in both control and mutant (I have them stored as=
> 16-bit RGB as well as 8-bit). As of now, I've been using the manual cell =
> counter plug-in through Fuji, but would like a way to specify an ROI and au=
> tomate this process. I have downloaded and tried the UCSB ITCN plugin for =
> automation, but it was not able to count cells closely packed together desp=
> ite a low threshold. If anyone can suggest a way to automate this (a new p=
> lugin or one contained into ImageJ), or help with the plugins that I have t=
> hat would be great.
>
> Thanks so much!!
> ~Francie
>
Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Processing and Analysis
Light Microscopy Facility
Max Planck Institute of Molecular Cell Biology and Genetics
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Germany
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(
[hidden email] )
Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany
+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
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