RGB Profiler values

Posted by Darren David Thomson on Jul 06, 2010; 12:58pm
URL: http://imagej.273.s1.nabble.com/RGB-Profiler-values-tp3687717.html

Hi

I'm currently running an experiment that looks at membrane channel  
internalisation over time.

i have a 2 channel image. RED with my membrane protein and GREEN with  
GFP (which stays inside the cell at all times due to being insoluable)

I did an RGB profile of the a line across the middle of the whole of  
cell of a merged G/R image and it gave me a great graph showing the  
RED peaks outside the cell (at time zero) and green peaking inside the  
cell.

I now need to get the number values out of this line that i have drawn  
across the cell. when i click MEASURE it only gives me grey scale  
values, not the separate green and red values.

My aim would be to measure the red values at the membrane compared to  
inside the cell over a time course of incubation.



Thanks for you time,

regards

Darren Thomson
BSc Physiology (hons)
ID:s0971727

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